WebTMB /ELISA stopped by acid is read at 450 nm (stopped) and 650 nm (not stopped). The addition of stopping agents can also increase the sensitivity of an ELISA. In the addition of stopping reagent ... WebTMB Super Slow One Component HRP Microwell Substrate. Click to view a larger image. Specifications; Documents; The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620 ...
Why stop reaction between HRP and TMB to read absorbance
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wtf is tmb2 (underbarrel attachment keybind) lol - Reddit
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