The tettag system
WebApr 23, 2014 · When this ChR2-TetTag system was combined with aversive experiences (foot shocks), light-driven excitation of the labeled neurons evoked an “artificial” fear response, demonstrating that the reactivation of an “engram” of fear memory was sufficient for memory recall in the hippocampus (Liu et al., 2012). WebJan 1, 2024 · This CreER T2-based system has a few advantages over the TetTag system, including a more precise temporal window for tagging (hours compared to days), permanent recombination-based modifications, a wide array of available Cre-based genetic tools, and the ability to delete endogenous genes (Guenthner et al., 2013; Reijmers and Mayford, …
The tettag system
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WebFeb 23, 2015 · The TetTag-hM 3 D q system to record and reactivate neuronal groups in the preoptic hypothalamus activated by a sedative dose of dexmedetomidine or during recovery sleep. (a) The AAV transgenes: the first contains the cfos promoter, which drives expression of … WebJan 1, 2024 · This CreER T2-based system has a few advantages over the TetTag system, including a more precise temporal window for tagging (hours compared to days), …
WebAn unbiased screen was developed using the c-Fos-inducible and doxycycline-regulated TetTag cFos system that labels cell nuclei with GFP permanently (Fig. 1a). This system was used previously to identify neurons involved in learning and memory (Matsuo et al., 2008). WebJun 2, 2016 · Tagging neural ensembles of contextual memories with the TetTag system. a, Schematic design of the TetTag system. b, Experimental design. Cells active in context A were tagged with GFP and cells active in context B, either 5h or 7d later, were labeled with ZIF immunohistochemistry. c, Representative examples of GFP, ZIF, DAPI and merged …
WebThis CreER T2 -based system has a few advantages over the TetTag system, including a more precise temporal window for tagging (hours compared to days), permanent … WebMay 18, 2011 · The TetTag mouse combines elements of the tetracycline-transactivator (tTA) system for transgene regulation with neuronal activity-induced activation of the c-fos promoter to tag activated neurons (Reijmers et al., 2007).
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WebThus, in its simplest form, many cells contain this molecular feedback loop that from publication: TetTag technology and the RAM system: activity-dependent neuronal marking from a circadian ... lyrica a controlled medWebThe initial system Gossen and Bujard developed is known as tetracycline off: in the presence of tetracycline, expression from a tet-inducible promoter is reduced. To use tetracycline as a regulator of gene expression, a tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16 ... lyrica ageWebJan 16, 2024 · Moreover, with use of the TetTag-hM3Dq system, we were able to verify the active involvement of pain- and anxiety-responsive LS neurons in the modulation of comorbid pain and anxiety behaviors. Under physiological conditions, the LS receives topographically organized inputs from the cortex and HPC and maintains appropriate … kirby carpet shampoo tube replacementWebTopics covered include types of traditional transgenic mouse models (knockout, knock-in, reporter lines), inducible systems (Cre-loxP, Tet-On, Tet-Off) and cell- and circuit-specific systems (TetTag, TRAP, DIO-DREADD). Additionally, we provide details on virus-mediated and siRNA/shRNA approaches, ... lyrica als drogeWebMay 23, 2016 · We confirmed these findings with the TetTag transgenic system, a non-invasive technique that allowed us to tag neurons active during the exploration of two … lyrica age love and hip hopWeb(C) Quantification of the TetTag labelled cells for the Primary somatosensory area (Kruskal Wallis test, group x %labelled cells, p < 0.001, Wilcoxon rank sum test for pairwise … kirby carpet shampoo system manualWebThe TetTag system. The TetTag transgenic mice contain two transgenes: one transgene expresses tTA by Fos promoter and the other transgene expresses dox-insensitive tTA* and tau-LacZ downstream of the tetO promoter. Neuronal activity can trigger activation of Fos promoter and lead to the expression of tTA, the transactivator of the tetO promoter. kirby castle leicester