2024 Strep tactin superflow high capacity

Strep tactin superflow high capacity

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August undefined, 2024

WebFor high-throughput, micro-scale purification of His-tagged proteins and versatile magnetocapture assays using His tags Strep -Tactin Superflow Plus For batch, gravity-flow, or FPLC purification of Strep -tagged proteins Ni-NTA HisSorb Strips and Plates For assays using 6xHis-tagged biomolecules Penta·His Alexa Fluor Conjugates WebStrep-Tactin ® Superflow or Strep-TactinXT Super-flow® column, respectively. Wash steps, each with 1 CV of Buffer W, were carried out until absorption at 280 nm reached a constant level. The elution of tar-get proteins from Strep-Tactin® resin was carried out by applying 0.8 CV, 1.4 CV and 0.8 CV of Buffer W

IBA LifeSciences Strep-Tactin Superflow high capacity …

WebGravity flow Strep-Tactin® Superflow® high capacity column Human Cells & Tissue Fetal Bovine Serum IBA Lifesciences Products Products Back Products Overview Antibodies/Markers Back Antibodies/Markers Overview Primary Antibodies Secondary Antibodies Antibody Supporting Products Human Cells & Tissue Back Human Cells & … WebThe new Strep-Tactin Superflow Plus has a 3-fold increased binding capacity. Table 1. Protein binding capacities of Strep-Tactin matrices Matrix Protein binding capacity Strep-Tactin Superflow Plus Up to 9 mg/ml Strep-Tactin Superflow Up to 3 mg/ml (~150 nmol @ 20 kDa) Strep-Tactin Magnetic Beads 200–300 μg/ml (10% suspension) chandler arizona subaru dealership

Strep-Tactin Superflow high capacity - Cedarlane

WebStrep-Tactin® Superflow® high capacity cartridge for one-step purification of recombinant Strep-tag® proteins with HPLC or FPLC. Human Cells & Tissue Fetal Bovine Serum IBA … WebStrep-Tactin Superflow high capacity is a 6% agarose coupled with the streptavidin variant Strep-Tactin and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. In comparison to Strep-Tactin Superflow, Strep-Tactin Superflow high capacity has a … WebThe Strep-tag® technology is a versatile protein purification, detection and immobilization platform. Strep-Tactin®XT provides binding affinity in low pM ranges while maintaining its binding... chandler arizona tax rate

IBA Lifesciences Strep-Tactin™XT Superflow™ High Capacity …

WebI found in the IBA material : When using Strep-Tactin® Superflow® High Capacity resin it may be advantageous to use 5-10 mM desthiobiotin to get the target protein eluted at higher concentration... WebNov 1, 2013 · Each sample was then applied to a 0.2-ml column containing Strep-Tactin® Superflow High Capacity. Bound target protein was eluted with desthiobiotin. Recoveries were determined and corresponding yields with respect to the amounts applied are given in %. ... A 1-ml Strep-Tactin® column was used to purify the Fab fragment from 1 ... harbor freight waukesha wisconsinWebCedarlane provides high quality research reagents for the life science community chandler arizona trash pickup

"WebStrep•Tactin ® resins have a binding capacity of 50–100 nmol/mL of resin (up to 3 mg for a 30 kDa protein), depending upon the size of the fusion protein. The dissociation constant (Kd) for Strep•Tag ® II is 1 µM. " - Strep tactin superflow high capacity

Strep tactin superflow high capacity

Strep-Tactin®XT Superflow® 50% suspension - Neuromics

Web1. Strep Tactin is not really compatible with many detergents (especially short chain, DM, DPC, ....) at "reasonable" concentrations (> 2 cmc) - in contradiction to the statement from IBA... WebThe recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification. Both Strep-Tactin® Superflow® resins • combine outstanding flow characteristics with ...

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WebBinding capacity depends on the Strep-Tactin® matrices (25 - 100 nmol/ml using standard Strep-Tactin® resins and up to 500 nmol/ml using Strep-Tactin® Superflow® High Capacity) as well as on the fused recombinant protein. Because of its small size, Strep-tag®II generally does not interfere with the bioactivity of the fusion partner. WebStrep-Tactin™XT Superflow™ high capacity is a 6% agarose coupled with the streptavidin variant Strep-Tactin™XT and applicable for purification of Strep-tag™II or Twin-Strep …

WebThe robust Superflow matrix allows flow rates up to 10 ml/min (1 ml cartridges) and 40 ml/min (5 ml cartridges), speeding the purification procedure and increasing throughput. The cartridges are quickly and easily connected to liquid chromatography systems or a syringe for manual purification. WebStrep-Tactin™XT Superflow™ high capacity is a 6% agarose coupled with the streptavidin variant Strep-Tactin™XT and applicable for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins. It allows the addition of denaturing agents such as urea during the purification procedure.

WebStrep-Tactin XT Sepharose contains the latest Strep-Tactin ligand, XT, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein than with the predecessor product, StrepTactin Sepharose High Performance (HP). Strep-Tactin XT Sepharose also enables purification under denaturing conditions. Web8 rows · Strep-Tactin ® Superflow ® high capacity cartridges contain a 6% agarose coupled with the ...

WebThe Strep-TactinXT Superflow high capacity resin provides 2 to 3 times higher binding capacity for Strep-tag proteins than standard Strep-TactinXT Superflow and therefore, …

WebThe Strep-tag® II can be fused to the protein at both the N- or C-terminus and binds with high selectivity to Strep-Tactin®. ... 2.4 Regeneration of Strep-Tactin®XT Superflow® High Capacity Resins 1. Wash the column with 15 CV of 10 mM NaOH for regeneration Always use freshly prepared 10 mM NaOH. Strep-Tactin®XT Superflow® resin cannot be ... chandler arizona time nowWeb8 rows · Strep-Tactin® Superflow® high capacity is a 6% agarose coupled with the streptavidin variant ... harbor freight water keyWebStrep-Tactin®XT Superflow is now also available as high capacity version combining the high affinity of Strep-Tactin®XT with a high protein binding capacity. Short Protocol of … chandler arizona senior living communitiesWebStrep-Tactin®XT Superflow is now also available as high capacity version combining the high affinity of Strep-Tactin®XT with a high protein binding capacity. Short Protocol of the Strep-Tactin®XT high capacity chromatography cycle Perform all operations at a temperature amenable to the stability of your recombinant protein (between 4°C and ... chandler arizona to phoenix arizonaWebStrep -Tactin Superflow gravity flow columns (IBA): Standard capacity, 1 mL and 5 mL bed volume. High capacity, 1 mL and 5 mL bed volume. 6. Strep -Tactin Superflow cartridges (IBA): Standard capacity, 1 mL and 5 mL bed volume. High capacity, 1 mL and 5 mL bed volume. 7. Strep -Tactin magnetic beads type 3 (IBA). 3 Methods harbor freight wausau wisconsinWebBinding capacity depends on the Strep-Tactin® matrices (25-100 nmol/ml using standard Strep-Tactin® resins and up to 230 nmol/ml using Strep-Tactin® Superflow® high … chandler arizona white pagesWebStrep-Tactin®XT Superflow® is used for purification of recombinant Strep-tag® II and Twin-Strep-tag fusion proteins under gravity flow and low pressure or FPLC. Bulk resin is … chandler arizona used furniture