Plvx-shrna2
Web靶向ERRα基因的慢病毒重组载体及其构建方法和应用,深圳市南山区疾病预防控制中心,202410931991.3,发明授权,本发明公开了一种靶向ERRα基因的慢病毒重组载体，包括pLVX‑shRNA1表达载体的基本序列、抗性基因序列、多克隆位点序列和靶向ERRα基因的寡核苷酸链序列；所述寡核苷酸链序列包括正义链序列 ... WebpLVX-Puro Lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Open in SnapGene Try SnapGene …
Plvx-shrna2
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WebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into ... WebMar 8, 2024 · The pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with efficient RNA interference in target cells.
WebJan 21, 2024 · The correct vector plasmid packaging virus was identified. The lentiviral shRNA-expressing plasmids pLVX-shRNA2-puro, Amp + (Biowit Technology, Shenzhen, China) were transfected with the packaging plasmids into 293T cells for lentivirus generation . The viral supernatants were harvested, and 293T cells were used to … WebFeb 1, 2024 · The targets of LACTB shRNA sequences were 5′-CCATGGTTGCTCTTGCCAA-3′ and 5′-GCCATAGTAGAGAGAGCTT-3’. Oligos containing the scrambled or non-targeted shRNAs were cloned into the pLVX-shRNA2-Luc-Puro vector labeled with luciferase. The oligo sequences are displayed in Table S1. Overexpressing or shRNA lentiviruses were …
WebDec 3, 2024 · The pLVX-shRNA2 and pLVX-IRES-ZsGreen1 plasmids (Clontech, USA) were selected as the lentiviral vectors for PC4 knockdown and overexpression, respectively. The target sequences for PC4 knockdown were PC4 sh1 GAAGGAACAGATTTCTGACAT; PC4 sh2 GACAGGTGAGACTTCGAGA; and PC4 sh3 GCAAAGTGCTAATTGATAT.
WebFast accurate construct design for all major molecular cloning techniques. Validate sequenced constructs using powerful alignment tools. Customize plasmid maps with …
WebAug 9, 2024 · Plasmids pLVX-shRNA2-m and PLVX-mcmv-ZsGreen1 were purchased from Biowit Technologies, Ltd. (China). pLVX-shRNA2-m was first digested with PstI and then filled in with Klenow. The larger fragment was then extracted and cleaved with BamHI. shovel kitchenWebJun 7, 2014 · The TβRII shRNA or negative control sequence was inserted into plvx-shRNA2 vectors (Clontech) using Bam H1 and Eco R1 restriction sites, packed according to the manufacturer’s instructions (Clontech, USA). To make lentiviral particles, ViraPower Lentiviral Expression System (Invitrogen) was used. shovel knight 16 bitWebpLVX-shRNA1 Vector Information. Clontech Laboratories, Inc. www.clontech.com Protocol No. PT4051-5 2 Version No. PR073571. Use. pLVX-shRNA1 is available in the Lenti-X™ shRNA Expression System (Cat. No. 632177). Before it can be transduced into target cells, the vector must be cotransfected into 293T cells with our Lenti-X HTX Packaging shovel knight 3ds dlcWebpLVX-shRNA2 can be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived … shovel key chainWebPlvx Shrna2 Puro Vector, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … shovel knight 3ds physicalWebSep 22, 2024 · IMPORTANT NOTE: NIH does not independently verify information submitted to the GTR; it relies on submitters to provide information that is accurate and not … shovel knight 3ds gameplayhttp://www.labbase.net/Supply/SupplyItems-4229347.html shovel knight 3ds pal cia