Phi29 polymerase rca
Web15. sep 2014 · Both plasmid DNA and MOSIC construct p378 RCA assays show how phi29 DNA polymerase amplifies a circular DNA mainly in double stranded form and how by … Web1. jan 2024 · Ramified RCA is performed as a one-pot reaction at constant temperature (30 °C for Phi29 DNA polymerase). The optimal reaction buffer will depend on both the …
Phi29 polymerase rca
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WebTempliPhi kits use isothermal rolling circle amplification (RCA) for the accurate, exponential amplification of circular DNA using Phi29 DNA polymerase. Random hexamer primers … WebProvided is a method for producing circular DNA in which a region sandwiched by a region Ha and a region Hb in circular double-stranded DNA has a linear DNA fragment entirely or partially substituted. In the circular double-stranded DNA, the region Hb is downstream of the region Ha. The linear DNA fragment is single-stranded or double-stranded DNA having …
WebPhi 29DNA 聚合酶来自于枯草杆菌噬菌体Phi29 中克隆出来的嗜温DNA 聚合酶，具有较高连续合 ... 80104L Phi 29 DNA Polymerase 1000U 有限使用商标许可 海狸纳米科技(苏州)有限公司声明对其开发的或者与其他单位合作开发的所有内容和服务拥有或者与合作者共同拥有全部 … Web26. aug 2024 · 3、RCA滚环扩增技术 原理：以环状 DNA 为模板，通过一个短的 DNA 引物（与部分环状模板互补），在 phi29 DNA 聚合酶催化下将 dNTPs 转变成单链 DNA，此单链 DNA 包含成百上千个重复的模板互补片段。 这种方法不仅可以直接扩增 DNA 和 RNA，还可以实现对靶核酸的信号放大，灵敏度达到一个拷贝的核酸分子，因此在核酸检测中具有很 …
Web1. máj 2009 · RCA has been performed in vitro beginning in 1989 using several DNA polymerases and different circular DNA templates 4, 5, 6, 7.The general principle is … WebΦ29 DNA polymerase is an enzyme from the bacteriophage Φ29. It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and …
Web14. nov 2005 · We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by >10 9-fold, using φ29 DNA polymerase.The principal …
Web8. apr 2024 · Summarizing the data presented, phi29 polymerase is less prone to inserting dUTPs* into DNA molecules during polymerization than Taq polymerase. Sulfo-cyanine3 … oxley hotel oxleyWeb乙型肝炎病毒在肝外组织中复制并影响血糖调节 摘要 目的: 研究肝外组织是否支持乙型肝炎病毒复制以及乙型肝炎的泛嗜性对血糖调节的影响。方法: 利用pcr法检测麻鸭血清中的鸭乙型肝炎病毒以筛选携带鸭乙型肝炎病毒的麻鸭，分组后进行静脉葡萄糖耐量实 oxley hotelsWeb7. sep 2010 · Multiply primed RCA of plasmidic DNA by φ29 DNA polymerase and chimerical DNA polymerases. ... R Johne, H Müller, A Rector, M van Ranst, H Stevens, Rolling-circle … jefferson county pay grade scalejefferson county pay gradesWebRecently, the bacteriophage phi29 DNA polymerase has been used for the efficient amplification of circular DNA viral genomes without the need of specific primers by a … oxley house planWeb7. mar 2024 · Yes, phi29-XT DNA Polymerase will work in the wild-type phi29 reaction conditions and it will generate comparable yield to the wild-type enzyme under these conditions. However, for phi29-XT DNA Polymerase, higher yields are expected when carrying out the reaction at 42°C. Links to this resource Related Products: phi29-XT RCA Kit oxley house woolwichWebThe Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries. On container: Subject to proprietary rights of Cytiva. jefferson county parks