Web1 apr. 2016 · Each 10 µL PCR reaction method included 5 µL of 2× MightyAmp Buffer Ver.3, 1 µL of 10× Additive for High Specificity, 0.2 µL of MightyAmp DNA Polymerase Ver. 3 (Takara, Shiga, Japan), 0.5 µL... WebFree essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics

MightyAmp DNA Polymerase Ver.3｜タカラバイオ株式会社

Web×MightyAmp Buffer Ver.2 (Takara, Otsu, Japan), 0.25 µM of each primer, and 1.25 units of MightyAmp DNA Polymerase (Takara). The PCR reaction and preparation of amplicon pool were performed by the method of Takahashi et al. [27] Bioinformatics analysis The determined 16S rDNA sequences were subjected to homology searching using … WebQ5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg ++ at final (1X) reaction concentrations and is … si session purdue

MightyAmp™ DNA Polymerase Ver - catalog.takara

WebThe PCR mixtures (50 μl) contained 25 μl 2×MightyAmp Buffer Ver.2, 1 μl of each primer (10 μmol/L), 1.0 μl MightyAmp DNA Polymerase, and 2.0 μl template DNA (10-200 ng), with nuclease-free water added to 50 μl. Web10 nov. 2024 · analyzing multiple environmental samples using MinION. The MinION sequencing of amplicons was adjusted by the different rounds of PCR performed. Soil fungi and bacteria were compared using ITS and 16S rRNA amplicons, respectively. Simultaneous amplicon analysis of multiple soil samples using MinION sequencing - … Webcomprising 25 μl 2× MightyAmp Buffer (TaKaRa), 1 μl (1.25 U) MightyAmp DNA Polymerase (TaKaRa), 0.3 μM of each primer, and 20–50 ng of genomic DNA. PCR was first incubated at 98 °C for 1 min and was followed by 35 cycles at 98 °C for 10 s, 60 °C for 15 s, and 68 °C for 60 s. The PCR products were visualized by agarose (1%) gel sisi mansour