Web13 apr. 2024 · Extracellular vesicles have shown good potential in disease treatments including ischemic injury such as myocardial infarction. However, the efficient production of highly active extracellular ... Web11 apr. 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting …
Hypoxia measurements in live and fixed cells using fluorescence ...
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Optimizing an Injectable Composite Oxygen-Generating …
Web11 apr. 2024 · Autotaxin (ATX), the key enzyme that generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC), is involved in tumorigenesis through the ATX-LPA axis and is regarded as a valuable target in tumor therapy. Hypoxia is a major feature of solid tumors and contributes to tumor development with striking alterations in the gene … WebImage-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties … Web21 okt. 2024 · Hypoxia is a common pathological process in ... 0.5 mL of diluted sample was mixed with 2 mL of 2 N Folin–Ciocalteu phenol reagent and 2.5 mL ... with double excitation wavelengths at 346 nm (blue) and 525 nm (green), respectively. Images were processed and fluorescence intensity was calculated using Image J software v 1.8.2. 4.4 ... broward residency form