Hieff clone enzyme
Web10912-A 2× Hieff Clone® MultiS Enzyme Premix 50 μL 100 μL 10912-B 500 bp Control Insert (25 ng/μL) 5 μL 5 μL 10912-C pUC 19 Control Vector, linearized (50 ng/μL) 5 μL 5 … WebNational Center for Biotechnology Information
Hieff clone enzyme
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WebHieff Clone™ Zero TOPO-TA Cloning Kit is developed by topo cloning technology, which is to achieve rapid cloning of inserts through the coupled isomerase (Topoisomerase) on the TOPO vector. This topo ta cloning kit provided by Yeasen contains TOPO-TA cloning vector and is suitable for taq-amplified PCR product WebHere's a list of top tips to keep in mind when designing your primers for seamless cloning, including some information specific to In-Fusion Cloning. The 3′ end of the primer should contain sequence that is specific to the target gene you are amplifying. This sequence should be 18–25 bases long and should ideally have a GC content between ...
WebEasy-A enzyme amplifies targets up to 10 kb from plasmid DNA and up to 5 kb from genomic DNA. FIGURE 1 The Easy-A PCR cloning enzyme produces amplification products that are ready to clone directly into the StrataClone PCR Cloning Kit vector arms (shown), or into T-/U-vectors (not shown) * U.S. Patent Nos. 6,734,293, 6,444,428, 6,183,997. Web1 de nov. de 2024 · Enzymes immobilized on solid supports have been certified to improve biocatalytic reusability. Hence, the immobilization of the polymers on solid ... amplified products were inserted into the recombinant plasmids pET22b-Linker1-SpyTag and pET22b-Linker2-SpyCatcher by Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co., Ltd ...
WebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。将 … http://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools
Web产品描述 . Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可 …
Web6 de jun. de 2024 · Restriction enzymes were purchased from New England BioLabs. Hieff Clone™ Plus One Step Cloning Kit was purchased from Shanghai Yeasen Biotech. All the primers used in this study were listed in Table S5. 3.3 Growth Condition of … dream music belmoreWebGolden Gate Assembly is another method of seamless cloning that exploits the ability of Type IIS restriction enzymes (such as BsaI-HF ® v2) to cleave DNA outside of the … engine stalls anti lock light onWebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, … dream music bandWebHieff Canace ® Gold 高保真DNA聚合酶. 10148ES60. 100 U. 2×Hieff Canace ® Gold 高保真酶预混液. 10149ES03. 1 mL. Hieff Clone ® 多片段一步法快速克隆试剂盒. 10912ES10. 10 T. Hieff Mut TM 定点突变试剂盒. 11003ES10. 10 T engine stalling when brakingWebDigest your DNA: Set up restriction digests for your donor and recipient plasmids. Because you lose some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend 1.5-2μg of … dream music exiterdream music earrapeWeb19 de jan. de 2024 · Then, AQP1 gene fragment (GenBank, NM_198098.3) was cloned into pGMLV-hTERT-MCS-EGFP using a Hieff Clone™ One Step Pcr Cloning Kit to generate pGMLV-hTERT-AQP1-EGFP plasmid. pGMLV-CMV-AQP1 ... All of the constructed plasmids were identified by restriction enzyme digestion analysis and DNA sequencing. … dream music clean