Web说明书 翻译. CROSS-REFERENCES TO RELATED APPLICATIONS [0001] The present ... The development of genome editing using engineered nucleases is the foundation for the idea that ex vivo gene correction constitutes a viable therapeutic strategy for both genetic and non-genetic diseases (Naldini, Nature Reviews. Genetics, 12, 301 ... WebsiPOOLs :一款特异性好的RNA干扰试剂,能够产出可靠的表型. siRNA pools (即 siPOOLs ) 是一款经过优化设计的高复杂性的RNAi试剂,包含了 30 条 siRNA 的混合物,经证明可有效消除脱靶效应,提高了结果的可靠性( Hannus et al., 2014 )。 Pack Hunter (pooling) 方法通过将当个 siRNA 的浓度稀释到刺激表型的阈值以 ...
Nuclease-mediated genome editing of primary cells and …
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