2024 Eea1 molecular weight

Eea1 molecular weight

Author: rgxv

August undefined, 2024

WebAntigen Molecular Weight: 162.46 Depositors Notes: Predicted Species Reactivity: Human Protein Atlas: Immunogen: Recombinant protein Gene: EEA1 Alternate Antibody Name: Alternate Gene Names: ZFYVE2 Alternate Antigen Name: Clonality: Monoclonal Myeloma Strain: SP2/0-Ag14 Epitope Mapped: No Uniprot ID: Q15075 Epitope Location or … WebEEA1 (G-4) is a mouse monoclonal antibody raised against amino acids 1-300 mapping at the N-terminus of EEA1 of human origin. PRODUCT 1. Each vial contains 200 µg IgG 1 …

EEA1, an early endosome-associated protein. EEA1 is a ... - PubMed

WebMar 1, 1999 · Abstract EEA1, an early-endosomal protein originally identified as an autoantigen, is essential for endocytic membrane fusion. It interacts with early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of the small GTPase Rab5. WebPCRP-EEA1-2H11 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-EEA1-2H11) Storage and … maxxfan deluxe with rain dome

SANTA CRUZ BIOTECHNOLOGY, INC. EEA1 (G-4): sc-137130

WebEarly endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane … WebEEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting … WebEEA1 (E-8): sc-365652 ... Molecular Weight of EEA1: 162 kDa. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. DATA SELECT PRODUCT CITATIONS 1. Huang, S.H., et al. 2013. Epidermal growth factor receptor-containing herr foods jobs

SANTA CRUZ BIOTECHNOLOGY, INC. EEA1 (E-8): sc-365652

WebMar 30, 2024 · Even though the depletion of VPS35 increased the size of the EEA1-labeled early endosomes, in line with SNX1 pattern, the endosomal actin patches were intact in the VPS35 KO cell line. ... (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented … WebMay 10, 2011 · The EEA1-containing high-molecular-weight complexes might constitute both EEA1 homodimer as well as EEA1 heterooligomers that contained other EEA1-interacting proteins. maxxfan deluxe troubleshootingWebBackbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name RAB7 (Homo … maxxfan cleaning

"WebMay 1, 2024 · Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. … " - Eea1 molecular weight

Eea1 molecular weight

SANTA CRUZ BIOTECHNOLOGY, INC. EEA1 (G-4): sc-137130

WebOct 13, 2024 · EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal … WebMolecular tethers, ... and EEA1 on the endosomes (Lupashin and Sztul, 2005; ... Molecular weight markers are indicated in kD. (G) Cell-surface ratio quantification (flow cytometry) showing reduced amounts of LAMP1Δ-RUSH at the plasma membrane after transient KO of PIP5K homologs and 35 min of biotin exposure.

Did you know?

WebFeb 4, 2000 · Early endosome antigen 1 (EEA1) is 170-kDa polypeptide required for endosome fusion. EEA1 binds to both phosphtidylinositol 3-phosphate (PtdIns3P) and to Rab5-GTP in vitro, but the functional role of this dual interaction at the endosomal membrane is unclear. WebImmunogen: Human EEA1 aa. 3-281 Application: Immunofluorescence (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Not Recommended) Target Molecular …

Web1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Notes; IP Use at 2-5 µg/mg of lysate. WB 1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Target. Function. ... EEA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using … WebCell Signaling Technology Inc wo2 mouse monoclonal Wo2 Mouse Monoclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.

WebMar 16, 2011 · CT subunit B Alexa Fluor ®-594 conjugate (CTxB-594) (Molecular Probes) was added to the wells at a final concentration of 1 µg/mL. After 1 or 3 h of toxin treatment the medium was removed and the coverslips were fixed with 3.7% formaldehyde in DMEM 10% BGS for 20 min. Coverslips were washed 3 times with 1× PBS and mounted onto a … WebEEA1 is a hydrophilic peripheral membrane protein present in cytosol and membrane fractions. It partitions in the aqueous phase after Triton X-114 solubilization and is …

WebEEA1 antibody can be used for detection of EEA1 by western blot at 0.06 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL 1: Cat. No. 1309 - Human Placenta Lysate PREDICTED MOLECULAR WEIGHT: 162 kDa Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method.

WebApr 10, 2024 · The mode of transport depends on the physical and chemical properties of the substances to be transported, including molecular weight, lipid solubility, plasma protein binding, and cerebral blood flow. Passive transport is limited to small-sized molecules that have sufficient lipid solubility such as gaseous oxygen and carbon dioxide … herr foods hainesportWebJun 2, 1995 · Membranes were incubated with affinity-purified human (Aff. pur. EEA1 Ab) or rabbit (Aff. pur. rabbit Ab) anti-EEA1 antibody ... although post-translational modification of EEA1 may contribute to the difference between its observed and predicted molecular weight. Rabbit Antiserum to Recombinant Protein Reacts with Cytoplasmic Vesicles and … herr foods locationsWebApr 10, 2024 · The lower molecular weight protein band, detected by the anti-human Alix antibody in bovine and swine cell lysates, may represent unspecific staining. ... like the early endosome-associated protein 1 (EEA1), recycling endosome-decorating Rab11, the late endosome marker Rab7, and the endolysosomal LAMP1 (lysosomal-associated … herr foods paWebEEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking ... Gene Name EEA1(Human) Molecular Weight 36.630kDa inclusive of tags; Add to Compare List. Creative BioMart. Phone: 1-516-855-7709. Email: [email protected]. herr foods warehouse in north east md hoursWebThe expected molecular weight of EEA1 (on the left-hand side) and molecular weight standards (on the right-hand side) are shown in the figure. (B) Fusion between early … herr foods corporate officeWebThis Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1. Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells. maxxfan inclusief montageWebEnter 197.13 into the Molecular Weight (MW) box Enter 10 into the Concentration box and select the correct unit (millimolar) Enter 10 into the Volume box and select the correct unit (milliliter) Press calculate The answer of 19.713 mg appears in the Mass box Tech Support Please see Inhibitor Handling Instructions for more frequently ask questions. herr foods logo